Extraction, Isolation and Structure Elucidation of a Bioactive Constituent from Chloroform Extract of <i>Moringa concanensis</i> Leaves

Moringa concanensis Nimmo. (Moringaceae) is a wild plant found in India. Traditionally, tribes used this as an antioxidant, anticancer, anticonvulsant, antimicrobial, antifertility, anti-inflammatory, antipyretic, and analgesic. In the present study, leaves of were subjected to successive solvent extraction with five different solvents increasing polarity, i.e., petroleum ether, chloroform, ethyl acetate, methanol, water, followed by phytochemical screening each extract. The study indicated presence terpenoids, steroids, cardiac glycosides, flavonoids, alkaloids, carbohydrates, proteins, tannins, phenolics. chloroform extract showed glycosides (cardenolides); therefore, RP-HPLC method was developed identify number constituents recrystallized isolate single component gain desired purity for further spectral analysis. Different data from UV-Visible, Infrared, Mass, Nuclear Magnetic Resonance spectroscopy analyzed isolated chemical constituent identified.